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Technical Articles
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There were 8 articles found in this category:
 Test Tube "popping off"when tube support arm set aside
During normal operation of the Facscan, Facsort, FacsCalibur, LSR and LSRII, the pressure inside the test tube (sample pressure) should be relieved when changing test tubes. If your test tubes are popping off the sample injection probe with some force ie. they are under pressure, this abnormal b ...
- DxP Optical Layouts
6 Color, 488/637 View Layout
- Monthly cleaning of FacsCalibur with Cytek Fluid Manager
The Monthly Cleaning Procedure should be performed at regular intervals or when FSC noise is excessive. This procedure cleans the supply lines and flow cell walls with 10% bleach, then rinses the beach out with deionized H2O. It is critical to bypass the sheath filter and to use a dedicated 4L b ...
- Considerations when Choosing a Cell Sorter
Utility Requirements: Higher powered 2 to 5W Ion lasers (Argon or Krypton) require 3-Phase electric power (and a transformer to drop the voltage to American standards), they also require water cooling (up to 20kW of heat per laser) with a rapid flow rate. The advantage of these lasers is that th ...
- Flow Cytometer Specifications
There are a number of specifications used to characterize flow cytometer performance. In order to compare the specifications of different cytometers, it is necessary to understand how these specifications impact the quality of data a researcher would obtain.
- Accurately Measuring Cytometer Sensitivity
“Minding your B’s and Q’s” Many cytometer manufacturers today rate the sensitivity of their flow cytometers using a fluorescence threshold. To perform this a set of beads with known Mean Equivalent Soluble Fluorescence (MESF) is run, and MESF vs. channel number is plotte ...
- Why Digital Electronics for Flow Cytometry?
The major flow cytometer manufacturers have converted from analog to digital electronics during the past 15 years. There are various blends of analog and digital electronics, but since main advantage of digital electronics is accurate logarithmic conversions, all the blends incorporate this feat ...
- Why Time Delay Calibration “Can Miss the Point… In time”
As the technology of flow cytometry advances, researches and clinicians wish to perform assays using more colors. A method to accomplish these multi-color assays is having multiple laser excitation sources. In most cases these multiple laser sources are spatially separated, creating emission pul ...
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