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  1. questionA Simple Method for Characterizing Cytometer Noise
    Noise level is an important parameter in determining efficacy in cytometer performance. Cytometer noise has both electronic and optical contributions, which together can decrease a cytometer’s low end resolution. We have developed a simple method using common blank/DIM polystyrene beads. U ...

  2. questionQ&b - Resolution of Dimly Fluorescent Particles: A Practical Measure of Fluorescence Sensitivity
    Flow cytometry is usually used to analyze subpopulations of cells, not simply to measure the mean fluorescence level of a mixture. Thus, resolution or coefficient of variation (CV) of dimly stained populations is the most appropriate measure of fluorescence "sensitivity." Methods used to measur ...

  3. questionTest Tube "popping off"when tube support arm set aside
    During normal operation of the Facscan, Facsort, FacsCalibur, LSR and LSRII, the pressure inside the test tube (sample pressure) should be relieved when changing test tubes. If your test tubes are popping off the sample injection probe with some force ie. they are under pressure, this abnormal b ...

  4. questionDxP Optical Layouts
    6 Color, 488/637 View Layout

  5. questionMonthly cleaning of FacsCalibur with Cytek Fluid Manager
    The Monthly Cleaning Procedure should be performed at regular intervals or when FSC noise is excessive. This procedure cleans the supply lines and flow cell walls with 10% bleach, then rinses the beach out with deionized H2O. It is critical to bypass the sheath filter and to use a dedicated 4L b ...

  6. questionConsiderations when Choosing a Cell Sorter
    Utility Requirements: Higher powered 2 to 5W Ion lasers (Argon or Krypton) require 3-Phase electric power (and a transformer to drop the voltage to American standards), they also require water cooling (up to 20kW of heat per laser) with a rapid flow rate. The advantage of these lasers is that th ...

  7. questionFlow Cytometer Specifications
    There are a number of specifications used to characterize flow cytometer performance. In order to compare the specifications of different cytometers, it is necessary to understand how these specifications impact the quality of data a researcher would obtain.

  8. questionAccurately Measuring Cytometer Sensitivity
    “Minding your B’s and Q’s” Many cytometer manufacturers today rate the sensitivity of their flow cytometers using a fluorescence threshold. To perform this a set of beads with known Mean Equivalent Soluble Fluorescence (MESF) is run, and MESF vs. channel number is plotte ...

  9. questionWhy Digital Electronics for Flow Cytometry?
    The major flow cytometer manufacturers have converted from analog to digital electronics during the past 15 years. There are various blends of analog and digital electronics, but since main advantage of digital electronics is accurate logarithmic conversions, all the blends incorporate this feat ...

  10. questionWhy Time Delay Calibration é─˙Can Miss the Pointé─Â In timeé─¨
    As the technology of flow cytometry advances, researches and clinicians wish to perform assays using more colors. A method to accomplish these multi-color assays is having multiple laser excitation sources. In most cases these multiple laser sources are spatially separated, creating emission pul ...